Development and Validation of a Simple HPLC PDA Method for the Simultaneous Analysis of 13- Docosenamide, Squalene and n-Tetracosanol-1 from the Leaf Extracts of Wagatea spicata

Nandini, Girish; Vikas, Vaidya

Journal of Stress Physiology & Biochemistry, Vol. 16 No. 3 2020, pp. 5-13 ISSN 1997-0838
Original Text Copyright (cc) 2020 by Nandini and Vikas

ORIGINAL ARTICLE

Development and Validation of a Simple HPLC PDA Method for the Simultaneous Analysis of 13- Docosenamide, Squalene and n-Tetracosanol-1 from the Leaf Extracts of Wagatea spicata

Girish Nandini and Vaidya Vikas

Ramnarain Ruia Autonomous College, L. Nappo Road, Dadar East, Matunga, Mumbai, Maharashtra 400019, (India)

*E-Mail: nandinigirish2011@gmail.com

Received February 29, 2020

Wagatea spicata (Dalzell) Wight [Moullava spicata (Dalzell) Nicolson] exhibits a diverse concentration of biologically active constituents such as Lupeol, Bergenin, Stigmasterol, Friedelin, n-Hexadecanoic acid, Palmitic acid, Gamma sitosterol,13docosenamide, squalene and n-Tetracosanol-1.
Present work focuses on development and validation of a precise, accurate and reproducible HPLC method for simultaneous quantification of three pharmacologically active phytochemical markers 13docosenamide, squalene and n-Tetracosanol-1 from the aerial parts of Wagatea spicata.

Key words: HPLC, 13-docosenamide, squalene, n-Tetracosanol-1, simultaneous, method development, method validation