ORIGINAL ARTICLE

Assessment of Induced Variability of Yield Contributing Traits in M1 Gamma Irradiated Germplasm of wheat (Triticum aestivum L.)

Sowing of radiated Germplasam of two selected virus free wheat cultivars Pirsabak-98 and Fisalabad-2000, were conducted in the department of Botany, Shaheed Benazir Bhutto University Sheringal during crop season November 2016 to study the impact of different doses of gamma radiation. Both cultivars were purchased from Tarnab Farm Peshawar and later on irradiated to 5, 10, 20 Krad at NIFA. (Nuclear institute for food and agriculture Peshawar). It was observed that inverse relationship was created in doses of 5, 10, 15 Krad against number of parameters like number spikelet per plant, number of spike per plant, spike length per plant, grain number per plant, 1000 seeds fresh weight, 1000 grain dry weight. Similarly the stated parameter means values gradually and continuously decreases with increasing of gamma irradiation concentration in both the hybrids pirsabak-98 and Fisalabas-2000 of wheat. It was also evaluated that high concentration of doses cause restrictive in the mean figure of all parameters which reflect that both cultivars pirsabak-98 and Fisalabad-2000 were sensitive high intensity of gamma radiation. Moreover there were also found a small number of mutant genotypes in Fisalabad-2000 at 15 krad due to genetic changeability similarly at 5 krad dose in both cultivar found pink color plant and sterile plant. As far as the effect gamma irradiation through of analysis for all the parameters were highly significant while due to the interaction doses and cultivar effects for the length of the spike was insignificant. Similarly the mean values for length of spike per plant, fresh weight of grain, and dry weigh of grain and number of grain per plant varietal effects were concluded as highly significant however the effects. Likewise in-significant outcome were obtained in the mean value due cultivar effect for fresh weight of 1000 seeds.