ORIGINAL
ARTICLE |
Transgenic lines of Arabidopsis with HSP101 gene in sense and anti sense orientations acquired resistance to hard heat shock (50° C 10 min or 45-47° C 1 hour) and to freezing (-4° C 2 hours) due to the preliminary 2 hour’s heating at 37° C. Thus, it was shown at the first time that the induction of the resistance to hard heat shock and freezing with mild heat shock is possible in the absence of HSP101 synthesis. Thellungiella with the genome to 95-97% identical to the genome of Arabidopsis did not have higher resistance to high temperature, but was significantly more resistant to freezing. It differed from Arabidopsis by several times higher contents of HSP101, HSP60 and HSC70. Contents of these HSPs in Arabidopsis increased as a result of hardening at 4° C what was accompanied by the increase of the resistance to freezing. It is supposed that the resistances to heat and cold shocks are dependent not only from HSP101, but also from other HSPs.
Key words: Arabidopsis, heat shock proteins, resistance to heat and cold, Thellungiella, transgenic plants