QueryDate : 2016-12-24
Cites : 0
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| Data source : Google Scholar QueryDate : 2016-12-24 Cites : 0 |
Background: In our early
publications was shown that electrophorhetic motility of erythrocytes
(EPME) is a high effective criteria of adaptation response. This
correlation is based on parallel development of adaptation syndrome and
activation of the main organism regulatory systems, such as
sympatoadrenalic and hypotalamo-hypophosial-adrenal ones.
Objective: study of the
influence of physical exercises and adrenaline injections on
electrophorhetic motility, membrahes phospholipids spectrum and
oxidative metabolism of the rats’ erythrocytes.
Methods: Rats were divided into
three equal groups. First group of animals was control (n=10; without
any manipulations). Rats of second group were subjected to physical
load in the form of a sailing duration of 15 minutes with a cargo
amounting to 10% of animal body weight (water temperature – 26-280C).
Rats of third group were intraperitoneally injected with adrenaline
hydrochloride (0.1 mg/kg). Blood sampling was made from the sublingual
vein in 15, 30, 60, 120 minutes and 24 hours after exposure. We
estimated the dynamics of the electrophorhetic motility of erythrocytes
(EPME), the phospholipid spectrum of erythrocytes membranes, the
concentration of malonic dialdehyde (MDA) and the state of the
glutathione system.
Results and conclusions: The
study suggests that red blood cell as a biological system is capable
for realization of stress response may develop a special “alarm
reaction” after action of the stress agent. This response initiates
activation of free radical processes and phospholipids profile in
erythrocyte membranes with reducing of its electronegativity. This
stage enhances the activity of the antioxidant system, is limiting the
development of lipid peroxidation processes, and leads to the
development of "adaptation stage" of the cellular system, coupled with
the restoration of the electronegativity of the membrane and the
mobilization of reserves of low molecular antioxidants, particularly
glutathione, as well as "structural antioxidant", due to the content of
readily oxidizable lipid (phosphoethanolamine) and lipids that are
resistant to oxidation (phosphatidylcholine, sphingomyeline).
