Bactericidal Influence of Silver Nanocomposites on Clavibacter michiganensis ssp. sepedonicus

Perfileva, А.I.; Zhivet’yev, М.А.; Gasisova, A.V.; Borovskii, G.B.; Graskova, I.А.; Sukhov, B.G.; Trofimov, B.А.

Journal of Stress Physiology & Biochemistry, Vol. 12 No. 2 2016, pp. 11-16 ISSN 1997-0838
Original Text Copyright (cc) 2016 by Perfileva, Zhivet’yev, Gasisova, Borovskii, Graskova, Sukhov and Trofimov

ORIGINAL ARTICLE

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QueryDate : 2016-12-24
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Bactericidal Influence of Silver Nanocomposites on Clavibacter michiganensis ssp. sepedonicus

Perfileva А.I.¹, Zhivet’yev М.А.¹, Gasisova A.V.¹, Borovskii G.B.¹, Graskova I.А.^1,2, Sukhov B.G.³, Trofimov B.А.³

¹ Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of Russian Academy of Sciences, PO 317, Irkutsk 664033, Lermontova St., 132, Russia
² The Irkutsk Scientific Center of Siberian Branch of Russian Academy of Sciences, Irkutsk 664033, Lermontova St., 130, Russia
³ A.E. Favorsky Irkutsk institute of Chemistry, Siberian Branch, Russian Academy of Sciences, Irkutsk, 664033, 1, Favorskogo St., 1., Russia

*E-Mail: alla.light@mail.ru

Received March 16, 2016

The results of studying the effect of silver nanocomposites received by chemical synthesis on the bacterium Clavibacter michiganensis ssp. sepedonicus (Cms) are presented. Cms is a Gram-positive bacterium, which causes one of the most dangerous potato diseases, ring rot. The effective alongside ecologically safe methods for combating Cms are lacking. As the agent, possible for application for this purpose, we investigated silver nanocomposites. For definition of nanocomposites influence on Cms we applied seedings to calculation the colony-forming units, after an incubation of bacteria with a nanocomposite in the distilled water (the grown poor environment) and on a nutrient medium. Influence of silver nanocomposites on cellular respiration which was estimated on oxygen absorption speed is also investigated. Thus, the obtained results demonstrate the occurrence of bactericidal effects of the substances under study, and favor the supposition on advisability of further research into the silver nanocomposites as the agents for agricultural recovery from the bacterial pathogens.

Key words: Clavibacter michiganensis subsp. sepedonicus, antibacterial activity, nanocomposites, silver