Rhythmical changes of a level nitric oxide (NO) in roots etiolated seedlings of pea (Pisum sativum L.) and influence of exogenous calcium

Glyan’ko, A.K.; Ischenko, A.A.

Journal of Stress Physiology & Biochemistry, 2014, Vol. 10, No. 4, pp. 56-66 ISSN 1997-0838
Original Text Copyright (cc) 2014 by Glyan’ko and Ischenko

ORIGINAL ARTICLE

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Rhythmical changes of a level nitric oxide (NO) in roots etiolated seedlings of pea (Pisum sativum L.) and influence of exogenous calcium

A.K. Glyan’ko, A.A. Ischenko

Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of Russian Academy of Sciences, PO 317, Irkutsk, 664033, Russia

*E-Mail: akglyanko@sifibr.irk.ru

Received September 16, 2014

Studied time dynamics (during 60 mines) a level oxide nitric (NO) in cross cuts of roots 2 – day etiolated seedlings of pea sowing (Pisum sativum L.) by use of fluorescent probe DAF-2DA and a fluorescent microscope depending on action exogenous calcium (Ca²⁺). During an exposition of seedlings on water, solution CaCl2 are shown fluctuation in level NO in roots – his increase and decrease that testifies to the certain rhythm in generation NO. Exogenous factors (Ca²⁺) change time dynamics of level NO in comparison with variant “water”. Ca²⁺chelate EGTA removes action exogenous calcium on rhythmical change of a level NO in roots. Results are discussed in aspect of close interference of signaling systems and molecules (Ca²⁺, NO, Н₂О₂).

Key words: Pisum sativum L., calcium (Ca2+), calcium chelate (EGTA), fluorescent probe, nitric oxide (NO)