Evaluation of the Effect of Oxidative Stress on Roots of Red Beet (Beta vulgaris L.)

Kolesnikova, E.V.; Ozolina, N.V.; Nurminsky, V.N.; Nesterkina, I.S.; Sitneva, L.A.; Lapteva, T.I.

Journal of Stress Physiology & Biochemistry, 2014, Vol. 10, No. 4, pp. 5-12 ISSN 1997-0838
Original Text Copyright (cc) 2014 by Kolesnikova, Ozolina, Nurminsky, Nesterkina, Sitneva and Lapteva

ORIGINAL ARTICLE

Data source : Google Scholar
QueryDate : 2016-12-24
Cites : 0

Evaluation of the Effect of Oxidative Stress on Roots of Red Beet (Beta vulgaris L.)

E.V. Kolesnikova, N.V. Ozolina, V.N. Nurminsky*, I.S. Nesterkina, L.A. Sitneva, T.I. Lapteva

Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of Russian Academy of Sciences, PO 317, Irkutsk, 664033, Russia

* Tel: +7 9148824647; fax +7 3952510754
*E-Mail: cell@sifibr.irk.ru

Received July 7, 2014

In the present investigation it was studied the effect of oxidative stress on root of red beet. The degree of stress exposure was assessed at various levels: in tissues of roots (conductometric method), on isolated vacuoles (time-lapse video recording), and transport activity of the vacuolar membrane enzyme, namely V-H⁺-ATPase (fluorescent probe method). The obtained results allow to conclude the negative effects of oxidative stress on different levels of plant organization. There were several effects: a significant increase of outflow of electrolytes from the tissues of roots, a great decrease of half-life time of isolated vacuoles, and a 2 times reduction of transport activity of tonoplast H⁺-ATPase. Thus, the methods used in this study can be applied to assess the intensity of the impact of oxidative stress on plants.

Key words: enzyme activity, electrolyte outflow, root of red beet, oxidative stress, stability of vacuoles