Journal of Stress Physiology & Biochemistry, Vol. 10 No. 2 2014, pp. 74-83 ISSN 1997-0838
Original Text Copyright (cc) 2014 by  Kumar, Sirhindi, Bhardwaj and Kumar



ORIGINAL ARTICLE
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QueryDate : 2016-12-24
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Brassinosteroids Denigrate the Seasonal Stress through Antioxidant Defense System in Seedlings of Brassica juncea L.

Sandeep Kumar*1, Geetika Sirhindi*1, Renu Bhardwaj2 and Manish Kumar1

1 Department of Botany, Punjabi University, Patiala – 147 002, Pb., India
2 Department of Botanical and Environmental Sciences, GNDU, Amritsar, Pb., India

*E-Mail: sandeep20684@gmail.com, geetika@pbi.ac.in

Received December 22, 2013


The present work has been undertaken to study the effect of exogenously application of 24-epiBL and 28-homoBL on soluble protein, proline contents and antioxidant defense system of Brassica juncea L. RLM 619 under the influence of seasonal stress. It was observed that 24-epiBL and 28-homoBL treatment enhance the soluble protein, dry weight and shoot length of B. juncea seedlings under seasonal stress. If seeds treated with the different concentrations (10-6, 10-8 and 10-10 M) of 24-epiBL and 28-homoBL revealed batter growth, protein and proline contents as compare to untreated seedlings. Similarly the activities of antioxidant enzymes SOD, CAT, APOX, DHAR, PPO and Auxinases were enhanced by the application of different concentration of both brassinosteroids, whereas MDA content was decrease with both brassinosteroids treatments. Then we have concluded that both brassinolides have the seasonal stress ameliorative properties in B. juncea seedlings grown under the influence of seasonal stress. This study culminates to the role of brassinolides as an anti-stress property for protection of plant from various types of stresses.

Key words:    Antioxidant enzymes, Brassica juncea, Brassinosteroids, ROS, Seasonal stress

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