Journal of Stress Physiology & Biochemistry, Vol. 9 No. 4 2013, pp. 310-318 ISSN 1997-0838
Original Text Copyright (cc) 2013 by  Azadi, Younesi, Tabatabaei



ORIGINAL ARTICLE
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Seed Germination, Seedling Growth and Enzyme Activity of Wheat Seed Primed under Drought and Different Temperature Conditions

Azadi M.S.1, Younesi E.2*. Tabatabaei S. A.3

1 Department of Agronomy and Plant Breeding, Islamic Azad University, Dezfoul Branch, Iran.
2 Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tehran, Karaj, Iran.
3 Agricultural and natural resources research center of Yazd, Iran

*E-Mail:  younesiehsan@ut.ac.ir


Received July 23, 2013


The study aimed was to determine the effects of drought stress (0, -4, -8, -12 bar) and osmopriming (-15 bar PEG 6000 for 15 at 24 h) on seed germination, seedling growth and enzyme activity at different temperatures  were  assessed in  the  laboratory  for  wheat. Results  showed  that  the highest  germination  percentage  (GP)  (94.33%),  normal  seedling  percentage  (NSP)  (92%), germination index (GI) (44.85) and seedling length (11.03 cm) were attained from osmo-priming in control  conditions.  Therefore,  seed  priming  with  PEG  6000  significantly  (p≤  0.01)  increased germination characteristics as compared to the unprimed seeds under drought stress. Also, osmopriming increased catalase (CAT) and ascorbate peroxidase (APX) as compared to the unprimed.

Key words:  Wheat, Osmo-priming, Germination characteristics, PEG 6000, enzyme activity

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