Journal of Stress Physiology & Biochemistry, Vol. 9 No. 4 2013, pp. 219-229 ISSN 1997-0838
Original Text Copyright (cc) 2013 by   Lyubushkina, Kirichenko, Grabelnych, Pobezhimova, Fedyaeva, Stepanov, Voinikov



ORIGINAL ARTICLE
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Role of Changes in Cell Fatty Acids Composition in the Increasing of Frost Resistance of Winter Wheat Suspension Culture

I.V. Lyubushkina1,2, K.A. Kirichenko1, O.I. Grabelnych1,2*, T.P. Pobezhimova1, A.V. Fedyaeva1, A.V. Stepanov1, V.K. Voinikov1

1 Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of RAS, Irkutsk, Russia
2 Irkutsk State University, Irkutsk, Russia

*E-Mail:  grolga@sifibr.irk.ru


Received July 11, 2013


Influences of low temperatures (4 and 8 ° С) on the frost tolerance and fatty acid compositions of  cells  in  a  winter  wheat  suspension  culture  have  been studied.  It has  been  found  that treatment of the culture with 4 °C (7 days) did not protect cells from subsequent freezing temperature action (-8 °С, 6 h) and was not accompanied significant changes in the fatty acid composition. On the contrary, the treatment of the culture with the temperature 8 °C (7 days) prevented the death caused by freezing temperature and the content of saturated fatty acids decreased: pentadecanoic acid (by 35,0%), palmitic acid (by 19,9%) and stearic acid (by 65,4%), and the content of α-linolenic acid increased by 94%. That was the cause of the double bond index (DBI) increase by 16%. The role of fatty acids composition changes in the process of increasing frost tolerance in plants are discussed.

Key words:   Triticum  aestivum  L.,  suspension  culture,  low  positive  temperatures,  freezing temperatures, cold hardening, fatty acids composition

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