Journal of Stress Physiology &
Biochemistry, Vol. 9 No. 4
2013, pp. 193-206 ISSN 1997-0838
Original Text Copyright (cc) 2013 by Perveen, Shinwari, Jan,
Malook, Rehman, Khan, Jamil
ORIGINAL ARTICLE
Data source : Google Scholar
QueryDate : 2016-12-24
Cites : 4
Low Temperature Stress Induced
Changes in Biochemical Parameters, Protein Banding Pattern and
Expression of Zat12 and Myb Genes in Rice Seedling
Salma Perveen1, Kamran
Iqbal Shinwari1, Mehmood Jan1, Ijaz Malook1,
Shafiq Rehman2, Murad Ali Khan3 and Muhammad Jamil1*
1 Department of Biotechnology
and Genetic Engineering, Kohat University of Science & Technology
(KUST), Kohat 26000, Pakistan 2 Department of Botany, Kohat University of Science &
Technology (KUST), Kohat 26000, Pakistan 3 Department of Chemistry, Kohat University of Science &
Technology (KUST), Kohat 26000, Pakistan
*E-Mail: dr.jamil@kust.edu.pk;
jamilkhattak@yahoo.com
Received July 10, 2013
Low temperature stress is one of
the main abiotic factors that reduce the productivity of many crops in
hilly areas around the world. In this study, rice seedling were exposed
to low temperature stress (control, 0°C, -2°C, -4°C and -6°C) for 2 hr
to observe its effect on two rice varieties (Basmati-385 and Shaheen
Basmati) through ion and proline contents, photosynthetic pigments,
total protein content, protein banding pattern
and expression of Zat12
and Myb
genes. Resulted showed different patterns of accumulation
of Na+ K+ and Ca+2 ions with the
decrease in temperature in both varieties. Proline
accumulation was gradually increased in
both varieties with the decrease in
temperature. Photosynthetic pigments
(Chlorophyll (Chl) a, b and
carotene) were negatively affected by low temperature
stress in both varieties, however, carotene content was much affected
than Chl a and b. Nonsignificant variation in protein contents was
observed at all levels of low temperature, but the effects of low
temperature stress on protein banding pattern of Basmti-385 and Shaheen
Basmati were different at different treatments. RT-PCR results
indicated that ZAT12 was upregulated by short term low
temperature stress while OsMYB show slight upregulation at -2°C as compared to the other
treatments. This study identified that ZAT12 and OsMYB function as a positive
regulator to mediate tolerance of rice seedlings at low temperature
stress.
