Journal of Stress Physiology &
Biochemistry, Vol. 9 No. 3
2013, pp. 98-112 ISSN 1997-0838
Original Text Copyright (cc) 2013 by Magdum
ORIGINAL ARTICLE
Data source : Google Scholar
QueryDate : 2016-12-24
Cites : 3
Effect of Agrobacterium
Induced Necrosis, Antibiotic Induced Phytotoxicity and Other Factors in
Successful Plant Transformation
Sandip S. Magdum
Amity Institute of Biotechnology, Amity
University, Noida 201303, India
*E-Mail: sandipmagdum@gmx.com Received February 16, 2013
Agrobacterium tumefaciens infection
and antibiotic wash are the critical steps of Agrobacterium mediated plant
transformation procedure, most time responsible for lower
transformation efficiency due to necrosis and phytotoxicity caused by
biotic stress of Agrobacterium and abiotic stress by antibiotics
respectively. Ammi majus Egyptian origin
medicinal plant and Pearl millet cereal grain crop were studied for
their stress responses to Agrobacterium
mediated transformation (AMT). Agrobacterium
strains LBA4404 (O.D.=0.6-0.8) and EHA105 (O.D.=0.2-0.4) were used for
transformation experiments to infect calli of Ammi majus and embryogenic calli of
Pearl millet respectively. Incase of antibiotic wash, Cefotaxime 500 mg
L-1 was used for LBA4404 infected Ammi
majus calli and Timentin 300 mg L-1 was used for EHA105 infected
embryogenic calli of Pearl millet.
Effects of Agrobacterium
infection, antibiotic and NaOCl washes on Agrobacterium removal and both
explants physiological changes during transformation experimental
procedures were studied. At the end of the experiments explants
survival efficiency of Ammi majus
and pearl millet were 8% and 5% respectively. Biotic and abiotic stress
factors responsible for lower efficiency were investigated with various
other factors and strategies were discussed which are need to be
considered for higher transformation events and target tissue survival.
