Journal of Stress Physiology &
Biochemistry, Vol. 9 No. 1
2013, pp. 229-245 ISSN 1997-0838
Original Text Copyright (cc) 2013 by Darwish, Lopez-Lauri and
Sallanon
ORIGINAL
ARTICLE
Data source : Google Scholar
QueryDate : 2016-12-24
Cites : 5
Study of photosynthesis
process in the presence of low concentrations of clomazone herbicide in
tobacco (Nicotiana tabacum)
Majd Darwish, Félicie
Lopez-Lauri, Huguette Sallanon
Laboratoire physiologie des fruits et légumes,
Université d’Avignon et des pays de Vaucluse 301, rue Baruch de Spinoza
BP 21239, 84916 Avignon cedex 9, France
Tel.: +33-4-9084-2200; fax: +33-4- 9084-2201
*E-Mail: majd.darwish@etd.univ-avignon.fr
Received October 25, 2012
The effect of
chemical residues of clomazone on photosynthetic processes has been
studiedby using several low concentrations of the herbicide
(0, 1, 0.1, 0.01, 0.001, 0.0001 and 0.00001) µM and
seedlings of two varieties of tobacco (Nicotiana tabacum L. cv. Virginie
vk51, Nicotiana tabacum,
L. cv. Xanthi). The content of photosynthetic
pigments, the parameters of the chlorophyll-a fluorescence
and the JIP-test were performed on an
adult leaf (AL) and a young leaf (YL), that gave a complementary
designto know the action's mode of clomazone on the plant
physiological processes. Clomazone reducedthe total chlorophyll
(a+b), carotenoids pigments (reduction in size antenna
pigments judged by an increase in the chlorophyll a/b
ratio) in young leaves more than adults leaves. The
maximal photochemical efficiency (Fv/Fm) of photosystem II (PSII)
decreased significantly in youngleavescompared to adult
leaves and in (Virginie) variety than (Xanthi) variety. Among
the parameters calculated of the JIP-test most affected by
the treatment, PIabs, 1-VJ, ABS/RC, DI0/RC, TR0/RC, ET0/RC,
ET0/ABS, which indicated acomparable effects of clomazone(1μM,
0.1µM, 0.01µM) between the two types of leaves and the varieties
used. More, the results showed that the concentration ( 1μM) was the
most effective amongthe other low concentrations used and
the (Virginie) variety ismore sensitive than the (Xanthi)variety.
We conclude that clomazone has probably two combined functions
(physiological, toxic) judged by the different
behavior of both types of leaves in the presence of the
herbicide.
