Smoke Priming, a Potent Protective Agent Against Salinity: Effect on Proline Accumulation, Elemental Uptake, Pigmental  Attributes and Protein Banding Patterns of Rice (Oryza Sativa)

Jamil, Muhammad; Malook, Ijaz; Parveen, Salma; Naz, Tayybah; Ali, Arshad; Ullah Jan, Sami; ur Rehman, Shafiq

Journal of Stress Physiology & Biochemistry, Vol. 9 No. 1 2013, pp. 169-183 ISSN 1997-0838
Original Text Copyright (cc) 2013 by Jamil, Malook, Parveen, Naz, Ali, Jan and Rehman

ORIGINAL ARTICLE

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QueryDate : 2016-12-24
Cites : 3

Smoke Priming, a Potent Protective Agent Against Salinity: Effect on Proline Accumulation, Elemental Uptake, Pigmental Attributes and Protein Banding Patterns of Rice (Oryza Sativa)

Muhammad Jamil¹*, Ijaz Malook¹, Salma Parveen¹, Tayybah Naz¹, Arshad Ali¹, Sami Ullah Jan¹ and Shafiq ur Rehman²

¹ Plant Stress Molecular Biology Lab, Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat 26000, Pakistan
² Department of Botany, Kohat University of Science and Technology, Kohat 26000, Pakistan

*E-Mail: dr.jamil@kust.edu.pk

Received October 23, 2012

The exogenous application of plant derived smoke solution through seed pre treatment is consider to create tolerance in the plant against salinity, for this purpose different dilution of plant derived smoke solution as 1:5000 Buhania, 1:1000 Buhania, 1:1000 Cymbopogon, 1:500 Cymbopogon were used against 0 mM, 50, 100 and 150mM NaCl solution in the medium. The effect was observed on total proline accumulation, heavy metals uptake, photosynthetic pigments and protein poly peptide bands intensity in two rice varieties as Basmati 385 (B-385) and Shaheen Basmati (S. Basmati). Proline concentration increases while chlorophyll “a” chlorophyll “b” and carotene level decreases with increasing salinity. On other hand zinc concentration increases while cadmium and lead concentration decrease in the crop under saline conditions. Intensity of protein polypeptides bands decreases gradually with increasing salinity level but plants from the seeds soaked with smoke solution alleviated the drastic affect of salinity, and intensity of bands was quite good by comparing with non primed seeds. It was concluded that seed priming with plant derived smoke solution show beneficial effect on crop to protect them from salinity.

Key words: Salinity, Smoke priming, rice, Proline rich proteins, Heavy metals, SDS-PAGE