Journal of Stress Physiology &
Biochemistry, Vol. 9 No. 1
2013, pp. 157-168 ISSN 1997-0838
Original Text Copyright (cc) 2013 by Rezaei, Ghanati,
Behmanesh, Safari and Sharafi
ORIGINAL
ARTICLE
Data source : Google Scholar
QueryDate : 2016-12-24
Cites : 6
Synergistic Accumulative
Effect of Salicylic Acid and Dibutyl Phthalate on Paclitaxel Production
in Corylus avellana Cell
Culture
Rezaei A.1, 2,* Ghanati F.3,
Behmanesh M.4, Safari M.3, Sharafi Y.1 1 Department of Horticulture, Shahed University, POB
18155–159, Tehran, Iran 2 Medicinal Plants Research Center, Shahed University, POB
18155–159, Tehran, Iran 3 Department of Plant Biology, Faculty of Biological
Sciences, Tarbiat Modares University, POB 14115–154, Tehran, Iran 4 Department of Genetics, Faculty of Biological Sciences,
Tarbiat Modares University, POB 14115–154, Tehran, Iran
*E-Mail: ayarezaei@gmail.com
Received October 22, 2012
Suspension cell
cultures of Corylus avellana
were challenged with salicylic acid and its combined use with dibutyl
phthalate solvent. Salicylic acid with concentrations of 12.5, 25 and
50 mg L–1 and 10% (v/v) dibutyl phthalate were used and
added on day 8 and 10 of subculture, respectively. The results showed
that growth, viability and protein content of cells were decreased by
the treatments, compared to control. In all treatments, hydrogen
peroxide content and lipid peroxidation rate of cells increased,
compared to those of the control cells. Activity of phenylalanine
ammonia-lyase increased by salicylic acid and, dibutyl phthalate
exaggerated effect of salicylic acid. While flavonoids content
decreased by the treatments, paclitaxel content increased
significantly. The extracellular paclitaxel was more affected, compared
to cell-associated paclitaxel and all treatments increased paclitaxel
release and specific yield compared to that of the control. The most
production of paclitaxel and specific yield of it were observed under
effect of combined use of salicylic acid (50 mg L–1) and
dibutyl phthalate, suggesting a synergistic accumulative effect.
