Journal of Stress Physiology &
Biochemistry, Vol.
8 No. 4
2012, pp. 289-301 ISSN 1997-0838 Original
Text Copyright (cc) 2012 by Axenov-Gribanov, Lubyaga, Shakhtanova,
Gurkov, Bedulina, Shatilina, Kondratyeva, Vereshchagina,
Timofeyev
ORIGINAL
ARTICLE
Data source : Google Scholar
QueryDate : 2016-12-24
Cites : 2
Determination of Lake Baikal
endemic amphipod Eulimnogammarus
verrucosus (Gerstf., 1858) thermal optima limits by changes in
its metaboliс markers
Axenov-Gribanov D. V.1,2,
Lubyaga J. А.1,2, Shakhtanova N. S.1,
Gurkov А. N.1, Bedulina D. S.1,2, Shatilina Zh. М.1,2, Kondratyeva Е. М.1, Vereshchagina K. P.1, Timofeyev М. А.1,2
1
Irkutsk State University. 664003 Irkutsk, K. Marx str., 1, Russia 2 Baikal research centre*. 664003 Irkutsk, Lenin str., 3-120
Russia
Tel.: +7 (3952) 600 893
*E-Mail: brc.contact@gmail.com
Received September 26, 2012
In present study the determination
of cellular metabolic markers under exposure of Lake Baikal endemic
amphipod Eulimnogammarus verrucosus
(Amphipoda, Crustacea) in a wide range of ambient temperatures was
carried out. The metabolic data was compared with species
termopreferendum data obtained in our previous studies. It was found
that exposure of amphipods under increased temperatures resulted in
increase of HSP70 content and lactate, activating of antioxidant
enzymes (catalase and peroxidase), as well as reduction of lactate
dehydrogenase and glutathione S-transferase activities. Exposure of E.
verrucosus at low temperatures resulted in decrease of HSP70 content,
increase of lactate level, peroxidase and lactate dehydrogenase
activation, and reduction of glutathione S-transferase activity.
It was noted that the most expressed changes of metabolism markers in
the species observed when temperature threshold was exceeded 11°C or
then temperature dropped below 4°C. The temperature range 4-11°C
represented the area of stability of cellular metabolic markers in E.
verrucosus and correlated with a preferred temperature limits,
previously obtained in behavioral experiments. Thus, for a first time
with Lake Baikal amphipods we showed that the zone of stability of
cellular metabolism in E. verrucosus closely related to their
behavioral thermal preference zones and is likely to reflect thermal
optima limits of the species.
