Journal of Stress Physiology &
Biochemistry, Vol.
8 No. 4
2012, pp. 276-288 ISSN 1997-0838
Original Text Copyright (cc) 2012 by Glyan’ko, Mitanova, Ischenko
ORIGINAL
ARTICLE
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QueryDate : 2016-12-24
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Influence of nitrogen
compounds on growth and the nitric oxide (NO) content in roots of
etiolated pea seedlings
Glyan’ko A. K.*, Mitanova N. B., and
Ischenko A. A.
State
Federal Budget Institution of Science Siberian Institute of Plant
Physiology and Biochemistry Siberian Division of the Russian Academy
Sciences; 664033, Irkutsk, p/b 317, Russia
The data on the influence of
different concentration of sodium nitroprusside (SNP), potassium
nitrate (KNO3), sodium nitrite (NaNO2) and
L-arginine on 3 days-old etiolated pea seedlings growth and nitric
oxide (NO) content in roots of these seedlings are obtained. It is
found that 24 h exposition of seedlings to SNP showed negative
influence on growth at a 4 mM dose; SNP at 0.05 mM doze stimulates
their growth and 0.1 mM doze did not influence on seedlings growth.
Using KNO3 negative influence on growth at the
same exposition was showed only at superhigh concentration – 20 mM.
Using NaNO2 negative influence on growth was shown
at a doze of 2 mM but concentration of 0.1 and 0.5 mM did not influence
on it. The exposition of seedlings to L-arginine caused growth
inhibition already at concentration of 0.5 mM and reached maximum at 4
mM doze. Determination of NO level in roots using fluorescent probe
DAF-2DA in variants with the greatest growth inhibition has showed,
that the maximal inhibition of growth in roots and highest level of NO
in roots in variants with SNP (4 mM) and NaNO2 (2 mM) was observed. In variants with KNO3 (20 mM) and L-arginine (4 mM) maximal
growth inhibition did not coincide with NO accumulation and was
observed after 30 min after the beginning of plants exposition and
further it was reduced in 24 h. Results are discussed in connection
with possible influence NO and participation of the investigated
connections in generation nitric oxide in roots of pea seedlings.
Key
words: L-arginine, growth, etiolated seedlings, pea, sodium
nitroprusside, potassium nitrate, sodium nitrite, nitric oxide
