Journal of Stress Physiology &
Biochemistry, Vol.
8 No. 4
2012, pp. 253-261 ISSN 1997-0838
Original Text Copyright (cc) 2012 by Ansari, Sharif-Zadeh
ORIGINAL
ARTICLE
Data source : Google Scholar
QueryDate : 2016-12-24
Cites : 25
Osmo and hydro priming
improvement germination characteristics and enzyme activity of Mountain
Rye (Secale montanum) seeds
under drought stress
Omid Ansari1*, Farzad
Sharif-Zadeh2
1 M.Sc. student., Agronomy and Faculty of
Natural Campus, University of Tehran, Karaj, Iran 2
Associate Prof, College of Agriculture and Natural Resources,
University of Tehran, Karaj, Iran
Impacts of various
concentrations of polyetylenglycol 6000 (PEG 6000)(0, -9, -11,
-13 and -15 bar) and hydro priming on Mountain Rye (secale montanum)
germination characteristic and enzyme activity were evaluated under
drought stress in the seed laboratory of Natural Resources Faculty,
University of Tehran, Karaj, Iran. Analyze of variance for hydro
priming showed that temperature × time of priming interaction was
significantly for germination percentage (GP), normal seedling
percentage (NSP), coefficient of velocity of germination (CVG),
seedling vigor index (SVI), coefficient of allometry (AC) and seedling
length (SL) under drought stress and for osmo priming showed that
Concentration of PEG × Temperature × Time of priming interaction was
significantly for all traits under drought stress. Results of
interaction effects for hydro priming showed that the highest GP (53%)
and NSP (23.5%) were attained from hydro priming for 16h at 15 ◦C
and the highest CVG (0.21) and AC (0.49) were attained from hydro
priming for 8h at 10 ◦C, also hydro priming for 8h at 15 ◦C increased
SL (3.15) as compared to the unprimed.Osmo priming with concentration
of -15 bar PEG for 24h at 15 ◦C increased GP (80.5 %), GI (17.9), NSP
(45 %), SVI (257.85) and SL (5.73 cm) and decreased MTG as compared to
the unprimed and other treatments of osmo priming. The highest CVG was
attained from concentration of -9 bar PEG for 24h at 10 ◦C. the highest
AC was attained from concentration of -9 bar PEG for 12h at 15 ◦C. Also
osmo and hydro priming increased catalase (CAT) and ascorbate
peroxidase (APX) as compared to the unprimed.