ORIGINAL ARTICLE

Data source :  Google Scholar QueryDate : 2016-12-24 Cites : 6

Effect of chelating agents and metal ions on nickel bioavailability and chlorophyll fluorescence response in wheat- An approach for attenuation of Ni stress

The objectives of the study are to analyze the physiological changes, biochemical alterations and attenuation of nickel toxicity effects in wheat seedlings under combined applications of Ni ions, metal chelators (EDTA/Citric Acid) and metal ions (Zn2+ /Mg2+). Wheat (Triticum aestivum L cv UP262) seedlings were grown hydroponically using different concentrations of Ni up to 7 days along with chelators and metal ions for study. The seedling growth was maximum with NiCl₂–Zn²⁺ (100μM) and minimum with NiCl₂–EDTA (100μM) treatments. Total chlorophyll content was maximum in the seedlings treated with NiCl₂-Zn2+ (100μM) and minimum in NiCl₂-EDTA (100μM) treatments. NiCl₂–EDTA (100μM) showed less Fo and Fm values and therefore, a trend in the decrease in OJIP transient indicates the maximum alteration of photochemical activity of PS-II in presence of NiCl₂–EDTA (100μM) treatment. Similar observation was found by NiCl₂ –EDTA (200μM) treatment where Fo and Fm values were noted to decline. High nickel content in roots of the seedlings was noted as compared to shoots.