QueryDate : 2016-12-24
Cites : 0
| ORIGINAL
ARTICLE |
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: Google Scholar QueryDate : 2016-12-24 Cites : 0 |
The present study was intended to reveal the differential isoesterase expression in leaves and roots of Vigna unguiculata L. in response to saline stress. Influence of NaCl (0, 3, 5, 7 and10 %) on seedlings of Vigna unguiculata
L. was analyzed. The seedlings were grown in the centre for a period of
30 days. The randomly collected whole plants were used as a source for
isoesterase isolation. The seedlings showed the maximum tolerance up to
10% of NaCl. The Poly acrylamide gel electrophoresis was performed by
Anbalagan method. The staining and fixation of the enzyme was performed
by the Sadasivam and Manickam method. Seedlings treated with various
concentrations of NaCl showed different banding profile based on the
concentration (0-10%) and duration (5-30 days) of salt treatment as
follows: 32 bands with five active regions, 56 bands with eight active
regions, 102 bands with eight active regions, 47 bands with seven
active regions, 64 bands with nine active regions and 84 bands with
nine active regions on 5th, 10th, 15th, 20th, 25th and 30th day
respectively. The changing pattern of isozymes during development may
be interpreted as evidence for differential timing of gene expression
correlated with the physiological stress. The results of the present
study concluded that the isoesterase patterns could serve as a useful biochemical marker of salinity.
