QueryDate : 2016-12-24
Cites : 1
| ORIGINAL
ARTICLE |
| | | Data source : Google Scholar QueryDate : 2016-12-24 Cites : 1 |
Isozymic
and protein diversity among five sugarcane varieties viz., Co 6304, Co
85019, Co 8371, Co 89003 and Co 91010 were studied to understand the
varietal interrelationship and to identify the biochemical marker for
the disease resistance and stress tolerance. The standard technique of
vertical gel electrophoresis PAGE was employed for size separation of
isozymes. The gel was stained with different staining solutions for
different isozyme systems viz. peroxidase, esterase, acid phosphatase,
alkaline phosphatase and proteins. Rf values of the banding profiles,
similarity index and variation between the varieties were analysed.
Among the four enzyme systems, peroxidase profile reveals the
difference between the disease resistant / susceptible and abiotic
stress tolerant / non tolerant varieties. The two isoperoxidase bands
with Rf values 0.62 and 0.66 showed their presence in disease resistant
and abiotic tolerant varieties. The presence of two marker bands (0.62,
0.66) of resistant and stress tolerant varieties suggest that the
variety Co 6304 may also be resistant to smut, wilt and moderately resistant to red rot and tolerant to drought.
