Journal of Stress Physiology & Biochemistry, Vol. 8 No. 2 2012, pp. 110-117 ISSN 1997-0838
Original Text Copyright (cc) 2012 by Alen'kina, Trutneva and Nikitina



ORIGINAL ARTICLE
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Change in the Content of Salicylic Acid and in the Activities of Phenylalanine Ammonia-Lyase and Catalase in Wheat Seedling Roots Under the Effect of Azospirillum Lectins

Alen'kina S.A., K.A. Trutneva, V.E. Nikitina

Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russian Federation

E-mail: alenkina@ibppm.sgu.ru


Received March 2  2012

We investigated the time course of changes in the endogenous content of salicylic acid, the ratio between the acid's free and bound forms, and changes in the activities of phenylalanine ammonia-lyase and catalase in wheat seedling roots under the effect of the lectins of two strains of the associative nitrogen-fixing bacterium Azospirillum: A. brasilense Sp7 and its mutant defective in lectin activity, A. brasilense Sp7.2.3. Differences in plant response to the action of the lectins from these two strains were established. On the basis of the obtained data, a model was proposed for lectin-assisted induction of resistance, according to which the lectin effect on the roots of seedlings results in accumulation of free salicylic acid, which inhibits catalase activity, ultimately leading to accumulation of hydrogen peroxide and to formation of induced resistance.

Key words: wheat seedling roots, salicylic acid, phenylalanine ammonia-lyase, catalase, Azospirillum brasilense, lectins



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