ORIGINAL ARTICLE

Data source :  Google Scholar QueryDate : 2016-12-24 Cites : 1

Studies on developmental variation of isoperoxidase and protein profile of Zea mays L.

Johnson M^1*, Janakiraman N², Chalini K¹, Narayani M¹, Kalaiarasi V¹

¹ Department of Plant Biology and Plant Biotechnology, St. Xavier’s College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.

² Department of Botany, St. Joseph’s College (Autonomous), Tiruchirappalli - 620 002, Tamil Nadu, India.

Tel: +91 97869 24334; Fax: + 91 462 2561765

^* E-mail: ptcjohnson@gmail.com

Received November 21, 2011

The present study was aimed to produce the isoperoxidase and protein marker for the maize cultivars from India and to find the isoperoxidase and protein expression on the various developmental stages. The leaf samples of Zea mays L. were harvested on 3^rd, 7^th, 11^th, 15^th, 19^th, 23^rd and 27^th d for electrophoretic analysis of isoperoxidase and protein. The total protein and isoperoxidase were isolated using the standard procedure described by Sadasivam and Manickam. Isozyme and protein separation was carried out using 10% non-denaturing polyacrylamide gel electrophoresis. The banding pattern suggested several similarities as well as presence of unique isoperoxidase and protein in each stage. These studies will be useful for the identification of the major proteins present at different developmental stages of Z. mays.

Key words: Electrophoresis, isoperoxidase, protein, Zea mays.