Oxidative Stress and Modulatory effects of the root extract of Phlogacanthus tubiflorus on the activity of Glutathione-S-Transferase in Hydrogen Peroxide treated Lymphocyte

Ramteke, A.; Hussain, A.; Kaundal, S.; Kumar, G.

Journal of Stress Physiology & Biochemistry, Vol. 8 No. 1 2012, pp. 5-15 ISSN 1997-0838
Original Text Copyright (cc) 2012 by Ramteke, Hussain, Kaundal and Kumar

ORIGINAL ARTICLE

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QueryDate : 2016-12-24
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Oxidative Stress and Modulatory effects of the root extract of Phlogacanthus tubiflorus on the activity of Glutathione-S-Transferase in Hydrogen Peroxide treated Lymphocyte

Ramteke A*, Hussain A., Kaundal S. and Kumar G.

Cancer Genetics & Chemoprevention Research Group, Department of Molecular Biology & Biotechnology, Tezpur University, Tezpur, Assam, India, 784028

Tel: +91 3712 267007 Ext 5407 Fax: +91 3712 267005/267006

E-mail: anand@tezu.ernet.in

Received November 21, 2011

Glutathione-S-transferase is one of the important enzyme systems that plays vital role in decomposition of lipid hydro-peroxides formed due to oxidative stress. In the present study GST activity increased in the lymphocytes treated with increasing concentration of H₂O₂, and decrease in the levels of GSH was observed. For similar treatment conditions LDH activity and MDA levels increased significantly leading to decrease in the cell viability. Treatment of lymphocytes with the root extract of Phlogacanthus tubiflorus (PTE) resulted in dose dependent decline in the GST activity and rise in GSH levels. LDH activity and MDA levels also declined that led to the increase of cell viability. Lymphocytes pre-treated with the PTE followed by H₂O₂(0.1 and 1%) treatment, decline in the activity of GST and increase in GSH levels was observed. Also we have observed decline in the activity of LDH and MDA levels in the lymphocytes for both 0.1 and 1% of H₂O₂ though the magnitude of change was higher in the lymphocytes pre-treated with the PTE followed with 1% of H₂O₂treatment. Significant increase in the cell viability for similar conditions was also observed. These findings suggest protective function of the root extracts might be through modulation of GST activity and levels of GSH and might find application in Chemomodulation in future.

Key words: Oxidative Stress / Glutathione-S-transferase / Phlogacanthus tubiflorus / Lymphocyte / Chemomodulation.