Journal of Stress Physiology & Biochemistry, Vol. 7, No. 4,
2011, pp. 301- 310. ISSN 1997-0838
Original
Text Copyright (cc) 2011 by Soghra Fallahi, Fatemeh Nabavizadeh, Seyed
Shahabadin Sadra, Ali Mohammad Alizadeh, Soheila Adeli, Hossein
Nahrevanian, Hamid Reza Sadeghipour and Gholamreza Hasanzadeh
The effects of Leptin on gastric ulcer due to physical and
psychological stress: Involvement of nitric oxide and prostaglandin E2
Soghra Fallahi a, Fatemeh Nabavizadeh a,*, Seyed Shahabadin Sadra, Ali Mohammad Alizadeh b, Soheila Adeli a, Hossein Nahrevanian c, Hamid Reza Sadeghipour a Gholamreza Hasanzadeh d
aDepartment of physiology, Tehran University of Medical Sciences, Tehran, Iran
bCancer Research Center, Tehran University of medical sciences, Tehran, Iran
cPasteur Institute of Iran, Tehran, Iran,
dDepartment of anatomy, Tehran University of Medical Sciences, Tehran, Iran
*E-mail: nabavizadeh2000@yahoo.com
Fax: +982166419484 Tel.: +982166419484
Received September 29, 2011
The present study aimed to
assess the effects of Leptin on physical and psychological stresses
inducing gastric ulcer. The potential role of endogenous nitric oxide
(NO) and prostaglandin E2 (PGE2) in the effects of Leptin on the
gastric ulcer in rats are also investigated. In this study, fifty-four
male Wistar rats were equally divided into 9 groups and Leptin (10
µg/kg, s.c.) was administered twice a day for 14 days in advance of
physical and psychological stress. Also, NG- nitro-l-arginine-methyl
ester (L-NAME, 10 mg/kg, an inhibitor of NO synthase) and indomethtacin
(5 mg/kg, in order to inhibit PG synthesis) were applied before Leptin
administration. Ulcer index, gastric acid secretion, NO
metabolites and PGE2 of stomach tissue suspension were all measured.
Results indicated that ulcer index and gastric acid secretion were
significantly decreased (p<0.05) in Leptin in compare with those of
single stress groups. Also, NO metabolites and PGE2 were increased
(p<0.05) in Leptin groups than both L-NAME and Indomethacin+Leptin
groups. Conclusively, data of this study emphasized the healing ability
of Leptin on gastric ulcer induced by physical and psychological
stresses involves the NO and PGE2 pathways.
Key words: Leptin, gastric ulcer, nitric oxide, PGE2, physical stress, psychological stress
