Journal of Stress Physiology & Biochemistry, Vol. 7, No. 4,
2011, pp. 241- 249. ISSN 1997-0838
Original Text Copyright (cc) 2011 by Johnson, Dooslin and Babu
Salt Stress responses on Protein Profile in Vigna unguiculata L.
Johnson, M*1, Dooslin Mary D2, and Babu A1
1 Department of Plant
Biology and Plant Biotechnology, St. Xavier’s College (Autonomous),
Palayamkottai – 627 002, Tamil Nadu, India
2 Department of Physics, Holy Cross College (Autonomous), Nagercoil, Kanyakumari Dist, Tamil Nadu, India – 629 004
Tel: + 91 97 86 92 43 34: Fax: + 91 46 22 25 619 87
*E-mail: ptcjohnson@gmail.com
Received September 22, 2011
The present study was aimed to elucidate the salt tolerant, salt inducible and salt sensitive protein of the Vigna unguiculata by Sodium Deodyl Sulphate – Poly Acrylamide Gel Electrophoresis. Seedlings of Vigna unguiculata
exposed to different environmental conditions exhibited a plethora of
physio-chemical responses. The seedlings treated with various
concentrations of NaCl at third day showed maximum of 85 bands, with
nine active regions and their MW-Rf values ranged from 0.012 to 0.891.
The seedlings treated with same experimental set up at fifth day showed
maximum number of 63 bands with eight active regions and their MW-Rf
values ranged from 0.108 to 0.891. On 5th day seedlings showed the
isoperoxidase expression with various sizes of bands. The soluble
protein showed a gradual increase during the initial stage and after
fifth day there was gradual decrease in their content.
Key words: Salt stress; Protein; SDS- PAGE; Isoperoxidase; Proline.
