TY  - JOUR
AB  - UV radiation is an important stress factor for plants which may result in damage to genetic system and cell membranes and several metabolic processes. UV-B has greater damaging effects on plants because the cell macromolecules such as DNA and protein having strong absorption at 280-320 nm. In the present study, UV-B stress was given to the seeds of Zea mays L. at two different time intervals (30 and 60 min) and that stressed seeds were grown under normal environment condition. The leaves of 10th and 20th day seedlings were collected for the analysis of protein and DNA profiles. Protein was analyzed by SDS-PAGE and DNA was analyzed by Restriction enzymes. When compared with control plants, increased numbers of protein and DNA bands were observed in UV-B treated plants. The present study concluded that the plant synthesis new proteins and DNA under UV treatment for the adaptation to the environmental conditions. These stressed proteins could be used as biomarkers for identification of stressed plant. Identification of quantitative trait loci for UV stress resistance may well be an effective analytical tool. This approach is promising, considering that saturated DNA marker maps are now available for both genetic model plants and crop plants.
AU  - John De Britto, A. 
AU  - Jeevitha, M.
AU  - Raj, T. L. S.
ET  - 2011
KW  - Restriction Enzymes
SDS-PAGE
Stress response
UV-B radiation
Zea mays L
M1  - 4
M3  - ORIGINAL ARTICLE
PY  - 2011
SN  - 1997-0838
SP  - 232-240
ST  - Alterations of protein and DNA profiles of Zea mays L. under UV-B radiation
T2  - Journal of Stress Physiology & Biochemistry
TI  - Alterations of protein and DNA profiles of Zea mays L. under UV-B radiation
UR  - http://www.jspb.ru/issues/2011/N4/JSPB_2011_4_232-240.pdf
VL  - 7
ID  - 59
ER  -