Journal of Stress Physiology & Biochemistry, Vol. 7, No. 4,
2011, pp. 197- 206. ISSN 1997-0838
Original Text Copyright (cc) 2011 by Zaytseva, Polejaeva, Svedentsov,
Solomina and Utemov
Efficiency of application original preservatives for conservation leukocytes at -40 °C
Zaytseva*1 O.O., Polejaeva1 T.V., Svedentsov1 E.P., Solomina1 O.N., Utemov2 S.V.
1 Laboratory
cryophysiology of blood, Physiology Institute at the Scientific Center
affiliated to the Ural branch of the Russian Academy of Sciences, Komi
Republic, 167982 Syktyvkar, Russia
2 Laboratory conservation of blood and tissues, Kirov
Research Institute of Hematology and Blood Transfusion,610027 Kirov,
Russia
*E-mail: ddic@yandex.ru
Received September 21, 2011
When frozen leukocytes
exposed to harmful factors of the complex, due to their complex
cellular structure and high metabolism. Cryopreservatives allow to
avoid damages, but most of which are toxic. The aim of the present
was to compare the efficacy of application of two non-toxic
solutions for conservation of leukocytes at -40°С for 1 day. It was
show that solution containing cryoprotector mixed action (a derivative
of urea) and antihypoxant (sodium fumarate) is most effective in
preserving the functional activity of leukocytes.
Key words: leukocytes, cryopreservative
