Journal of Stress Physiology & Biochemistry, Vol. 7, No. 4,
2011, pp. 187- 196. ISSN 1997-0838
Original Text Copyright (cc) 2011 by Rakhra, Sharma, Singh and Vyas
Expression analysis of boiling-stable protein (BsCyp) in
response to drought, salt and osmotic treatments in drought tolerant
and susceptible cultivars of Triticum aestivum
Gurmeen Rakhra1, Arun Dev Sharma1*, Jatinder Singh2, Dhiraj Vyas3
1PG Department of Biotechnology, Lyallpur Khalsa College, G T Road, Jallandhar-144001,Punjab,India,
2Dept of Molecular Biology and Biochemistry, Guru Nanak Dev University, Punjab, India.
3Dept of Biodiversity, Indian Institute of Integrative Medicine (IIIM), Jammu, India.
*E-mail: arundevsharma@hotmail.com, arundevsharma47@rediffmail.com
Received September 20, 2011
The effect of osmotic,
drought and salt stresses on the induction of boiling-stable proteins
were studied in C-306 (drought tolerant) and HD-2004 (drought
susceptible) cultivars of Triticum aestivum.
Western blotting analysis, using polyclonal antibodies raised against a
20 kDa A. thaliana cyclophilin, revealed the induction of a
cross-reacting band (40 kDa, BsCyp) in cv. 306. However no substantial
expression was observed in drought sensitive cv. HD-2004, indicating
its role in water stress adaptation. Thermostability studies of BsCyp
in cv. 306 revealed that BsCyp was stable and can be detected even
after 20 min of boiling treatment under all the stresses. Based upon
these observations the possible role of BsCyp in water stress tolerance
is discussed.
Key words: Boiling-stable proteins, cyclophilin, water stress, wheat
