Journal of Stress Physiology & Biochemistry, Vol. 6 No. 4
2010, pp. 5-11 ISSN 1997-0838
Original Text Copyright (cc) 2010 by Kataria, Kataria, Joshi
ORIGINAL
ARTICLE
Data source : Google Scholar
QueryDate : 2016-12-24
Cites : 2
Fuelling of TCA cycle in hepatic cells Marwari goat during ambient
temperature associated stress
Kataria N.1*, Kataria A.K.2, Joshi A.1
1 Department of Veterinary
physiology, College of Veterinary and Animal Science, Rajasthan
University of Veterinary and Animal Sciences, Bikaner – 334 001,
Rajasthan, India 2 Apex Centre for Animal Disease Investigation, Monitoring
and Surveillance, College of Veterinary and Animal Science, Rajasthan
University of Veterinary and Animal Sciences, Bikaner – 334
001, Rajasthan, India *Phone , 0091 – 151- 2546399 *Email-nalinikataria@rediffmail.com
Received July 16, 2010
The present study was launched to assess the effect of extreme ambient
temperature associated stress on fuelling of TCA cycle in hepatic cells
of Marwari goat. Based on the
fact that whenever a hepatocyte needs fuel for TCA cycle, the activity
of enzyme glutamate dehydrogenase (GD) increases making
alpha-ketoglutarate available for TCA cycle, 600 apparently healthy Marwari
goats of either sex, between 6 months to 3 years of age were screened
and blood samples were collected during moderate, cold and hot ambient
temperature periods to determine the serum glutamate dehydrogenase
enzyme and glucose concentration. The mean value of serum GD was
significantly (p≤0.05) higher during cold and hot ambient temperature
periods in comparison to overall moderate mean value. However, the rise
was greater in cold (2.20 times) than hot ambient temperature (1.19
times). The serum GD activity was higher in male and younger animals.
Serum glucose concentration showed a reverse trend as compared to serum
GD activity. The results indicated that in cold condition associated
stress the fuelling to TCA cycle was more than moderate and hot ambient
temperature periods. Serum GD activity was also found related with
glucose homeostasis. Further the study has shown that variations in the
enzyme levels are not always pathological and while interpreting
clinical data, a clinician must consider these variations.
key words: Ambient temperature /cold/
glucose/glutamate Dehydrogenase/ hot/Marwari goat
