Journal of Stress Physiology & Biochemistry, Vol. 6 No. 2
2010, pp. 10-18 ISSN 1997-0838
Original Text Copyright (cc) 2010 by Tushar, Manoj and Sushama
ORIGINAL
ARTICLE
Data source : Google Scholar
QueryDate : 2016-07-13
Cites : 6
Evaluation and Genetic Polymorphism
studies of Jatropha (Jatropha curcus)
for Water Stress Tolerance
Borse Tushar*, Kulkarni Manoj and Chaphalkar Sushama
Vidya Pratishthan’s School of
Biotechnology Vidyanagari, Bhigwan Road, Baramati 413133 INDIA. Phone: 91-2112-239388; 91-2112-239386 *Email- thbvsbt@gmail.com
Received February 12, 2010
Jatropha (Jatropha curcus)
is an alternative resource for biodiesel. To boost the rural economy in
sustainable manner it is estimated that 30 Million hector plantation
may replace current use of fossil fuel. Although Jatropha has an
inbuilt ability to grow under water limited conditions, scanty
information is available about natural genetic variation for water
stress tolerance. Three local genotypes from Pune district were
collected and initially screened by imparting artificial stress using
PEG – 6000. Seedlings were subjected to increasing concentration of PEG
– 6000 (30, 60, 90, 120 and 150 gm/l) to study effect on growth
parameters. The root growth, number of secondary roots, true leaf
expansion at morphological level and palisade mesophyll height, xylem
vessel expansion at anatomical level showed drastic negative impact as
compared to control. It is worth to note that local germplasm
performance was categorized into susceptible group as compared to
tolerant genotype [Chattisgadh Selection] indicating need for genetic
improvement. These genotypes were further studied at molecular level
with RAPD and ISSR markers to amplify genetic variation. Polymorphic
bands from Chattisgadh selection genotype are being evaluated for their
usefulness as markers for water stress tolerance.
key words: Jatropha curcus, water stress
tolerance, PEG, Anatomical,
RAPD
