Journal of Stress Physiology & Biochemistry, Vol. 5, No. 3,
2009, pp. 4-12 ISSN 1997-0838
Original Text Copyright (cc) 2009 by Kumar, Kumar, Bora, Amb
ORIGINAL
ARTICLE
Data source : Google Scholar
QueryDate : 2016-12-24
Cites : 3
Photosynthetic, biochemical and enzymatic investigation of Anabaena fertilissima in response
to an insecticide -
hexachloro-hexahydromethano-benzodioxathiepine-oxide.
Kumar Nirmal J.I*1, Kumar Rita N.2, Bora Anubhuti1
and Amb Manmeet Kaur 1
1P.G. Department of
Environmental Science and Technology, Institute of Science and
Technology for Advanced Studies and Research (ISTAR), Vallabh
Vidyanagar -388 120, Gujarat, India. 2Department of Biosciences and Environmental Science,
Natubhai V Patel College of Pure and Applied Sciences (NVPAS), Vallabh
Vidyanagar -388 120, Gujarat, India. E-mail: istares2005@yahoo.com
Received May 7, 2009
A study on the heterocystous, nitrogen fixing cyanobacterium, Anabaena fertilissima
was carried out to investigate the effect of an organochlorine
insecticide (hexachloro-hexahydro-methano-benzodioxathiepineoxide,
called as endosulfan) at different concentrations of 3, 6 and 12 μg*ml-1
on the photosynthetic pigments - Chl-a, Carotenoids and
Phycobiliproteins-phycocyanin, allophycocyanin and phycoerythrin,
stress metabolites such as carbohydrates, proteins, amino acids,
phenols and enzyme activities-nitrate reductase and glutaminesynthetase. The insecticide- Endosulfan showed to be deleteriously
affecting the activities in the cyanobacterium. As early as the 4th
day, chl-a and carotenoids reduced by 38% and 20% respectively. Thephycobiliproteins declined by 60%, 64% and 28% with respect to
Phycocyanin, Allophycocyanin and Phycoerythrin. Moreover, Endosulfan
adversely depleted the cellular activities, leading to a marked
decrease inthe carbohydrates, proteins, phenols and amino acids and
enzymes-nitrate reductase and glutamine synthetase. Despite of
deleterious effects of Endosulfan on the cyanobacterium Anabaena fertilissima, a unique regenerating ability in presence of the
insecticide was observed by the end of 12 days in the lower doses of
insecticide.
