TY  - JOUR
AB  - Late embryogenesis abundant (LEA) proteins are speculated to protect against water stress deficit in plants. An over expression system for mungbean late embryogenesis abundant protein, emv2 was constructed in a pET29a vector, designated pET-emv2 which is responsible for higher expression under the transcriptional/translational control of T7/lac promoter incorporated in the Escherichia coli BL21 (DE3). Induction protocol was optimized for pET recombinants harboring the target gene. Overexpressed EMV2 protein was purified to homogeneity and the protein profile monitored by SDS-PAGE.
AU  - Rajesh, S.
AU  - Raveendran, M.
AU  - Sivakumar, P.
AU  - Manickam, A.
ET  - 2008
KW  - LEA protein
Overexpression
Vigna
desiccation
M1  - 3-4
M3  - ORIGINAL ARTICLE
PY  - 2008
SN  - 1997-0838
SP  - 9-16
ST  - Overexpression Analysis of emv2 gene coding for Late Embryogenesis Abundant Protein from Vigna radiata (Wilczek)
T2  - Journal of Stress Physiology & Biochemistry
TI  - Overexpression Analysis of emv2 gene coding for Late Embryogenesis Abundant Protein from Vigna radiata (Wilczek)
UR  - http://www.jspb.ru/issues/2008/N3/JSPB_2008_3_9-16.pdf
VL  - 4
ID  - 94
ER  -