1Centre for Plant
Molecular Biology, Tamil Nadu Agricultural University,
Coimbatore-641003, India 2National Research
Centre for Soybean, Indore-452001, Madhya Pradesh, India 3Institute of
Biological Sciences, Faculty of Science, University of Malaya, 50603
Kuala Lumpur, Malaysia
Received July 7, 2008
Abstract - Late embryogenesis abundant (LEA) proteins are
speculated to protect against water stress deficit in plants. An over
expression system for mungbean late embryogenesis abundant protein, emv2
was constructed in a pET29a vector, designated pET-emv2 which is
responsible for higher expression under the
transcriptional/translational control of T7/lac promoter incorporated
in the Escherichia coli BL21 (DE3). Induction protocol was optimized
for pET recombinants harboring the target gene. Overexpressed EMV2
protein was purified to homogeneity and the protein profile monitored
by SDS-PAGE.
