THE INFLUENSE OF MONOIODACETATE ON THE THERMOTOLERANCE OF CLAVIBACTER MICHIGANENSIS SSP. SEPEDONICUS AND SACHAROMYCES CEREVISIAE

Rymareva, E. V.; Rikhvanov, E. G.; Torgashina, M. A.; Perfileva, A. I.; Kopytchuk, V. N.; Varakina, N. N.

Journal of Stress Physiology & Biochemistry, Vol. 4, No. 2, 2008, pp. 4- 13. ISSN 1997-0838
Original Text Copyright (cc) 2008 by Rymareva, Rikhvanov, Torgashina, Perfileva, Kopytchuk, Varakina

ORIGINAL ARTICLE

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THE INFLUENSE OF MONOIODACETATE ON THE THERMOTOLERANCE OF CLAVIBACTER MICHIGANENSIS SSP. SEPEDONICUS AND SACHAROMYCES CEREVISIAE

Rymareva E.V., Rikhvanov E.G., Torgashina M.A., Perfileva A.I., Kopytchuk V.N., Varakina N.N.

Siberian Institute of Plant Physiology & Biochemistry SD RAS. 664033, Irkutsk, POBox 1243, Lermontov st., 132, Russia
e-mail: elenar@sifibr.irk.ru

Received Mart 30, 2008
Received in revised form April 10, 2008

Abstract – To search the antiseptic agents capable to decontaminate the plants from pathogens the combined effect of moderate heat shock (45^oC) and glycolisis inhibitor monoiodoacetate (MIA) on survival of potato pathogen Clavibacter michiganensis ssp. sepedonicus (Cms) and yeast Saccharomyces cerevisiae was studied. Under optimal temperature cultivation (26^oC) MIA had no toxic effect on S. cerevisiae but decreased viability of Cms. The lethal effect of MIA significantly increased during heat treatment at 45^oC. MIA in the range from 0.1 to 1 mM decreased the thermotolerance of Cms and S. cerevisiae cells in 10-10000 folds in dependence from time of treatment. A minimal concentration of MIA capable to affect the thermotolerance was 0.1 and 0.3 mM for S. cerevisiae and Cms, respectively. The effect of MIA on Cms and yeast survival during heat shock was stronger in logarithmic phase than in stationary ones.

Key words: monoiodacetate, thermotolerant, Clavibacter michiganensis ssp. sepedonicus, Sacharomyces cerevisiae