Journal of Stress Physiology & Biochemistry, Vol. 2, No. 1,
2006, pp. 28-34.
Original Text Copyright (cc) 2006 by Svedentsov, Tumanova, Chtcheglova,
Solomina
ORIGINAL
ARTICLE
Data source : Google Scholar
QueryDate : 2016-12-24
Cites : 3 +2
CONSERVATION
LEUKOCYTES IN THE
CONDITIONS OF CRYOANABIOSIS (-400С)
Laboratory cryophysiology
of blood, Komi Institute of Physiology of Russian Academy of Sciences,
167982 Syktyvkar, Russia
Received November 3, 2005
Trying to meet the requirements in leukocytes the clinicists are facing
the pressing problem of conservation of cells in a functionally valid
condition which is possible in deep cryoanabiosis (-196°C). This
conservation method is not, for the number of reasons, available for
the purpose of a wide use. The aim of the present was development of an
efficient, readily available and economical method for conservation of
leukocytes at a moderately low temperature of -40°C. It was shown that
use of this method allows for conservation of blood nuclear cells in a
functionally valid condition for a period of 30 days.
Key
words:
leukocytes, cryoanabiosis, cryopreservative,
exponential programme for freezing