Journal of Stress Physiology & Biochemistry, Vol. 1, No. 1,
2005, pp. 15-20.
Original Text Copyright (cc) 2005 by Graskova, Antipina, Potapenko,
Voinikov
ORIGINAL
ARTICLE
Data source : Google Scholar
QueryDate : 2016-12-24
Cites : 0 + 6
PATHOGEN IMPACT ON
THE ACTIVITY DYNAMICS OF POTATO SUSPENSION CELLS EXTRA-CELLULAR
PEROXIDASE
Received May 13, 2005
Received in revised form July 6, 2005
Abstract— Changes in the activity
of extracellular peroxidases were
measured in cell suspension cultures of potato infected by Clavibacter
michiganensis subsp. sepedonicus (Spieck. et Kotth.) Skapt et
Burkh.
The total extracellular peroxidases activity of the resistant potato
variety was higher than that of the sensitive variety both before and
after infection. The enzyme of the resistant variety had a рН optimum
of 6.2, while that of the sensitive variety was 5.4. Extracellular
peroxidases of the sensitive potato variety were activated 10 minutes
after infection, and displayed highest activity 1.5-2 hours later. In
the resistant variety, peroxidase activity rose sharply in the first
minutes of infection, and second peak of activity occurred 1.5-2 hours
later. The increase of extracellular peroxidases activity of the
sensitive potato variety under pathogenesis is connected with the
change of genome expression and synthesis of proteins. The increase of
enzyme activity of resistant potato variety in the first moments of
infection is not related to proteins synthesis and is apparently
conditioned by the change of kinetic parameters.
